Biocompatibility of a nickel-free austenitic steel assayed by osteoblastic MC3T3-E1 cells

✍ Scribed by M. Mölders; A. Fischer; M. Wiemann

• Book ID: 102500544
• Publisher: John Wiley and Sons
• Year: 2002
• Tongue: English
• Weight: 230 KB
• Volume: 33
• Category: Article
• ISSN: 0933-5137
• DOI: 10.1002/mawe.200290010

✦ Synopsis

Abstract

The in vitro biocompatibility of the nickel‐free austenitic steel (brand name P2000) characterized by extreme strength, high ductility and superior corrosion resistance was tested. As the material appears promising for hard tissue implant development we employed osteoblastic cells (MC3T3‐E1) as test specimens. Cells growing directly on this metal were undistinguishable from control cells on cell culture tested plastic material with respect to morphology and growth parameters. P2000 can, therefore, be classified as a biocompatible material. In line with that application of bone morphogentic protein 2 (BMP‐2) increased expression of alkaline phosphatase as it does under control conditions. However, the slope of the dose response curve was diminished, indicating partially impaired BMP‐2 signaling on P2000. Since P2000 contains nitrogen and may release alkalizing amounts of NH~3~ we compared steady state intracellular pH (pHi) of cells on P2000 vs. controls, but were unable to find any differences. Further studies are needed to understand how P2000 can influence cellular functions of directly contacting cells.