𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Biochemical genetic studies of cycloheximide resistance inNeurospora crassa

✍ Scribed by Henry Rothschild; John Germershausen; Sigmund R. Suskind

Book ID
104784926
Publisher
Springer
Year
1975
Tongue
English
Weight
857 KB
Volume
13
Category
Article
ISSN
0006-2928

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✦ Synopsis

Genetic analysis of a number of cycloheximide-resistant mutants of Neurospora crassa has shown that resistance is Controlled by several genes. Two of these appear to be located on linkage group V. Resistance to the antibiotic is dominant in wild-type-mutant heterokaryons. Two types of cyeloheximide-resistant mutants were isolated: one type exhibited colonial morphology only when grown in the presence of cyeloheximide and the other type maintained normal morphology even at high concentrations of the antibiotic. Reconstitution experiments with supernatant solutions and 80S monosomes prepared from wild-type and resistant mutant strains indicated that the property of cyeloheximide resistance most likely is assoeiated with the ribosomes. No eleetrophoretic or serological differences were found between the ribosomal proteins of the wild-type and resistant mutants.

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