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Biochemical characterization of the cholesterol-dependent growth of the NS-1 mouse myeloma cell line

โœ Scribed by Jan-Kan Chen; Tetsuji Okamoto; J.Denry Sato; Gordon H. Sato; Don B. McClure

Book ID
115892101
Publisher
Elsevier Science
Year
1986
Tongue
English
Weight
702 KB
Volume
163
Category
Article
ISSN
0014-4827

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Development of a serum-free medium for growth of NS-1 mouse myeloma cells and its application to the isolation of NS-1 hybridomas
โœ Tomoyuki Kawamoto; J.Denry Sato; Anh Le; Don B. McClure; Gordon H. Sato ๐Ÿ“‚ Article ๐Ÿ“… 1983 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 771 KB

In this study, we have devised a hormone-supplemented, lipid-enriched serum-free medium, designated KSLM, that supports the growth of NS-1 mouse myeloma cells and have studied its applicability to the efficient isolation of antibody-producing hybridomas formed from the fusion of NS-1 myeloma cells a