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An alternative method for the isolation of NS-1 hybridomas using cholesterol auxotrophy of NS-1 mouse myeloma cells

โœ Scribed by Yoshinari Myoken; Tetsuji Okamoto; Teruhiko Osaki; Masafumi Yabumoto; Gordon H. Sato; Kazuaki Takada; J. Denry Sato

Book ID
112800131
Publisher
Society for In Vitro Biology
Year
1989
Tongue
English
Weight
375 KB
Volume
25
Category
Article
ISSN
1475-2689

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Development of a serum-free medium for g
Development of a serum-free medium for growth of NS-1 mouse myeloma cells and its application to the isolation of NS-1 hybridomas
โœ Tomoyuki Kawamoto; J.Denry Sato; Anh Le; Don B. McClure; Gordon H. Sato ๐Ÿ“‚ Article ๐Ÿ“… 1983 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 771 KB

In this study, we have devised a hormone-supplemented, lipid-enriched serum-free medium, designated KSLM, that supports the growth of NS-1 mouse myeloma cells and have studied its applicability to the efficient isolation of antibody-producing hybridomas formed from the fusion of NS-1 myeloma cells a