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Biochemical characterization of the Ca2+release channel of skeletal and cardiac sarcoplasmic reticulum

✍ Scribed by Gerhard Meissner; Eric Rousseau; F. Anthony Lai; Qi-Yi Liu; Kristin A. Anderson

Publisher: Springer
Year: 1988
Tongue: English
Weight: 498 KB
Volume: 82
Category: Article
ISSN: 0300-8177
DOI: 10.1007/bf00242517

No coin nor oath required. For personal study only.

✦ Synopsis

Rapid mixing-vesicle ion flux and planar lipid bilayer-single channel measurements have shown that a high-conductance, ligand-gated Ca2+ release channel is present in 'heavy', junctional-derived membrane fractions of skeletal and cardiac muscle sarcoplasmic reticulum. Using the release channel-specific probe, ryanodine, a 30S protein complex composed of polypeptides of Mr approximately 400,000 has been isolated from cardiac and skeletal muscle. Reconstitution of the complex into planar lipid bilayers has revealed a Ca2+ conductance with properties characteristic of the native Ca2+ release channel.

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