Bioaffinity purification of NADP+-dependent dehydrogenases: Studies with alcohol dehydrogenase from Thermoanaerobacter brockii

Purification by dye-ligand chromatograph

Purification by dye-ligand chromatography of an NADP-dependent enzyme, 6-phosphogluconate dehydrogenase from lamb's liver

✍ A. Carne 📂 Article 📅 1982 🏛 Elsevier Science 🌐 English ⚖ 249 KB

Purification of the NADP'-dependent 6-phosphogluconate dehydrogenase from lamb's liver using dye-ligand chromatography on Matrex Gel Red-A enabled a 2.5fold increase in yield of the enzyme and reduced the preparation time of a preivous scaled-up version of the published small-scale method.