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Binding to retinoblastoma pocket domain does not alter the inter-domain flexibility of the J domain of SV40 large T antigen

✍ Scribed by Christina K. Williams; Sivaraja Vaithiyalingam; Michal Hammel; James Pipas; Walter J. Chazin


Book ID
116178958
Publisher
Elsevier Science
Year
2012
Tongue
English
Weight
961 KB
Volume
518
Category
Article
ISSN
0003-9861

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With the aim of forming the β€˜lock-washer’ conformation of the origin-binding domain of SV40 large T antigen in solution, using structure-based analysis an intermolecular disulfide bridge was engineered into the origin-binding domain to generate higher order oligomers in solution. The 1.7β€…Γ… resolutio