The technique of equilibrium dialysis has been used to study water and salt binding to egg albumin, to human carbon monoxide hemoglobin, and to bovine serum albumin. The salts used were CsC1, KC1, NaCl, LiC1, Gu.HC1, NaBr, Cs2SO4, KzS04, Na2S04, Li2SO4, and Gu2S04. The amount of water bound by prote
Binding studies by dialysis equilibrium: A description of an accurate and rapid technique
โ Scribed by Rapier H. McMenamy
- Publisher
- Elsevier Science
- Year
- 1968
- Tongue
- English
- Weight
- 396 KB
- Volume
- 23
- Category
- Article
- ISSN
- 0003-2697
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๐ SIMILAR VOLUMES
## Equilibrium dialysis (1, 2) is a proved technique for obtaining quantitative data concerning the reversible interaction between a dialyzable species and a macromolecule. Upon proper treatment of the binding data, it is possible to determine the equilibrium constant for the formation of the com
Equilibrium dialysis (ED) is one of the most frequently used approaches to investigate drug binding, where the major drawbacks are the time to reach equilibrium (varying between 6 and 24 h), a long assay preparation time and complexity of automation. A rapid equilibrium dialysis (RED) device has rec
## Abstract Various factors influencing the protein binding of DAโ8159 to 4% human serum albumin (HSA) were evaluated using an equilibrium dialysis technique at an initial DAโ8159 concentration of 5 ยตg/mL. It took approximately 8 h incubation to reach an equilibrium between 4% HSA and an isotonic p