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Binding properties of biotinylated epidermal growth factor to its receptor on cultured cells and tissue sections

✍ Scribed by Eva Spitzer; Maria de Los Angeles; Rolando Perez; Richard Grosse

Publisher
John Wiley and Sons
Year
1989
Tongue
English
Weight
630 KB
Volume
41
Category
Article
ISSN
0730-2312

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✦ Synopsis

A biotinylated derivative of murine epidermal growth factor (EGF) was prepared by covalent attachment of the terminal amino group of EGF to N-biotinyl-e aminocaproyl-N-hydroxysuccinimide. The stoichiometry of biotin incorporation was in the range of one biotin moiety per EGF molecule. The biotinylated EGF (biotinyl-eaproyl-EGF, BioEGF) binds to EGF receptors on intact Ehrlich ascites carcinoma (EAC) cells with an affinity similar to that of native EGF and displays the same mitogenic activity as EGF in a soft agar test system with normal rat kidney (NRK) cells. BioEGF was visualized on cultured cells and tissue sections of a head and neck tumour by commercial streptavidin/avidin detection systems. Cytochemical analyses of certain tumour forms can be easily performed using the BioEGF probe.

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