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Binding of substrates to cytochrome P450 enzymes: Mathematical artifacts in the application of difference spectrophotometry

✍ Scribed by Shakunthala Narasimhulu

Publisher
Elsevier Science
Year
1990
Tongue
English
Weight
703 KB
Volume
187
Category
Article
ISSN
0003-2697

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✦ Synopsis

Investigation of the difference absorption spectrum has been a common method for studying many aspects of hemoproteins such as cytochrome P450. The difference spectrophotometric technique, as applied in studies on binding of substrates to cytochrome P450, has resulted in certain mathematical artifacts which have been mistaken for real changes in binding equilibria. The purpose of this paper is to point out these artifacts.

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