Binding of protein to polystyrene in solid-phase immuno assays
β Scribed by A.J. Pesce; D.J. Ford; M. Gaizutis; V.E. Pollak
- Book ID
- 113125433
- Publisher
- Elsevier Science
- Year
- 1977
- Weight
- 455 KB
- Volume
- 492
- Category
- Article
- ISSN
- 0005-2795
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
A solid-phase protein assay (SPA) for the determination of protein concentrations in the nanogram range is described. The technique is based on biotinylation of immobilized protein on the solid phase of a microtiter plate and quantitation of the protein-biotin complexes by peroxidase-coupled avidin.
The binding parameters, the affinity constant (Ka) and binding capacity (Q), of a protein possessing ligand-protein complexes with a high dissociation rate (Sex Steroid Binding protein from Bufo arenarum) were determined using a solid-phase method. The technique is based upon the adsorption of the s