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The use of protein A in solid-phase binding assays: a comparison of four radioiodination techniques

✍ Scribed by George L. Mayers; Jim Klostergaard

Book ID
118898906
Publisher
Elsevier Science
Year
1983
Tongue
English
Weight
648 KB
Volume
57
Category
Article
ISSN
0022-1759

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A solid-phase protein assay (SPA) for the determination of protein concentrations in the nanogram range is described. The technique is based on biotinylation of immobilized protein on the solid phase of a microtiter plate and quantitation of the protein-biotin complexes by peroxidase-coupled avidin.