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Binding of ligands by proteins: a simple method with sephadex gel

✍ Scribed by Masaaki Hirose; Yasuyuki Kano

Book ID
115748872
Publisher
Elsevier Science
Year
1971
Weight
182 KB
Volume
251
Category
Article
ISSN
0005-2795

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πŸ“œ SIMILAR VOLUMES

A simple inexpensive method for obtainin
A simple inexpensive method for obtaining sharp, narrow starting zones in columns packed with sephadex gels
✍ Uday V. Koppikar; M.R. Chivate; S.V. Koppikar πŸ“‚ Article πŸ“… 1977 πŸ› Elsevier Science 🌐 English βš– 338 KB

A simple, effective, economic method of obtaining sharp, narrow, uniform starting zones in ordinary laboratory glass columns packed with Sephadex gels has been described. A combination of Millipore filter and annulus and spiral layering of samples starting from the center outward has been found to g

Ligand binding to protein measured by ge
Ligand binding to protein measured by gel filtration on a dispersion column
✍ J.Tze-Fei Wong; Lloyd S. Porter πŸ“‚ Article πŸ“… 1983 πŸ› Elsevier Science 🌐 English βš– 465 KB

A double conical gel-permeation column is described, which permits an efficient dispersion of solutes in passage. It makes possible a facile determination of ligand binding to protein on the basis of the differential gel permeation of these molecules. The system has been applied, using Sephadex G-25