Binding of indole-3-acetic acid to human serum albumin and competition with l-tryptophan
β Scribed by Alessandro Bertuzzi; Geltrude Mingrone; Alberto Gandolfi; Aldo V. Greco; Severin Ringoir; Raymond Vanholder
- Book ID
- 117075587
- Publisher
- Elsevier Science
- Year
- 1997
- Tongue
- English
- Weight
- 487 KB
- Volume
- 265
- Category
- Article
- ISSN
- 0009-8981
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## Abstract When drugβprotein binding data are evaluated thermodynamically standard free energy (ΞGΒ°), standard enthalpy (ΞHΒ°) and standard entropy (ΞSΒ°) are usually estimated from association constants __(K__~a~) derived from binding data obtained at only two temperatures. Estimation of ΞHΒ° involv
Described in this paper is a rapid, isocratic assay for serum indole-3-acetic acid (IAA). The sample preparation involves only protein precipitation using sulfosalicylic acid, and the sensitivity of amperometric detection is in the picogram range. The chromatographic analysis time is approximately 4