## Abstract Binding constants and binding site sizes for the interactions of the polyamines spermine (+4), spermidine (+3), and putrecine (+2) with helical DNA have been determined as a function of ionic conditions and temperature by equilibrium dialysis using ^14^Cβlabeled polyamines. In addition,
Binding of calcium to glycosaminoglycans: An equilibrium dialysis study
β Scribed by Graeme K. Hunter; Kenneth S. Wong; John J. Kim
- Book ID
- 115707866
- Publisher
- Elsevier Science
- Year
- 1988
- Tongue
- English
- Weight
- 604 KB
- Volume
- 260
- Category
- Article
- ISSN
- 0003-9861
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The technique of equilibrium dialysis has been used to study water and salt binding to egg albumin, to human carbon monoxide hemoglobin, and to bovine serum albumin. The salts used were CsC1, KC1, NaCl, LiC1, Gu.HC1, NaBr, Cs2SO4, KzS04, Na2S04, Li2SO4, and Gu2S04. The amount of water bound by prote
In recent years there has been great interest in using hexammine cobalt(II1) as an isovalent analogue of spermidine. Hexammine cobalt(II1) qualitatively mimics spermidine in many of its physical effects on nucleic acids. Quantitatively, however, there are often large differences in the effects of th