Binding of Active Cyclosporins to Cyclophilin A and B, Complex Formation with Calcineurin A
β Scribed by B. Ryffel; G. Woerly; M. Murray; H.P. Eugster; B. Car
- Book ID
- 115573313
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 474 KB
- Volume
- 194
- Category
- Article
- ISSN
- 0006-291X
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The interaction of cyclosporin \(A\) and cyclosporin derivatives with cyclophilins \(A, B\), and \(C\) has been investigated by means of fluorescence measurement techniques. Since Trp-121 of cyclophilin \(A\) is in close contact with bound cyclosporins and changes its fluorescence emission upon bind
Seven purified cyclosporine (CsA) metabolites were analyzed for binding to cyciophilin and to a 50 kDa protein purified from a JURKAT cell line. In addition, the potency of the seven metabolites, relative to CsA, was obtained using a primary mixed lymphocyte culture (MLC) suppression assay. CsA, M1,