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Binding of 125I-labelled human αinterferon to human lymphoid cells

✍ Scribed by Knud Erik Mogensen; Marie-Thérèse Bandu; Franloise Vignaux; Michel Aguet; Ion Gresser

Publisher
John Wiley and Sons
Year
1981
Tongue
French
Weight
749 KB
Volume
28
Category
Article
ISSN
0020-7136

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✦ Synopsis

Abstract

To investigate the binding of interferon to human lymphoid cells, we purified human α interferon and radio‐labelled it with iodine −125. Binding at 4°C could be saturated and was inhibited by unlabelled interferon; it was specific for cells of human origin. Dissociation constants for the complex of interferon and receptor site were of the order 10^−9^–10^−11^ M. All human cells tested showed such binding. Occupation of these high‐affinity sites, at 37°C, was compared with the inhibition of cellular growth due to interferon. The most sensitive cell line (Daudi) gave a complete biological response with only a fraction of its sites occupied. Evidence of two sites was found for a line (P3HRI) showing intermediate sensitivity. A relatively insensitive line (Raji) showed no response when all its high‐affinity sites were occupied.

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