Displacement chromatography of proteins was successfully carried out in both hydrophobic interaction and reversed-phase chromatographic systems using low-molecular weight displacers. The displacers employed for hydrophobic displacement chromatography were water soluble, charged molecules containing
Bifunctional Adsorbents for Hydrophobic Displacement Chromatography of Proteins
โ Scribed by Ruoh-Chyu Ruaan; Andy Yang; Darren Hsu
- Book ID
- 111522733
- Publisher
- American Institute of Chemical Engineers
- Year
- 2000
- Tongue
- English
- Weight
- 58 KB
- Volume
- 16
- Category
- Article
- ISSN
- 8756-7938
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
In contrast to high molecular weight polyelectrolyte displacers, the efficacy of low molecular weight displacers are dependent on both mobile phase salt and displacer concentration. This sensitivity to the operating conditions opens up the possibility of carrying out selective displacement where the
Alginate use in displacement chromatography as a displacer has been studied. The experiments showed that untreated alginate is the basis of potential displacer for dsplacement chromatography, but needs to be cleaved into smaller chains. AIginate treated with ultrasound, which cleaves alginate into s