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Hydrophobic displacement chromatography of proteins

โœ Scribed by Abhinav A. Shukla; Khurram M. Sunasara; Randall G. Rupp; Steven M. Cramer

Publisher
John Wiley and Sons
Year
2000
Tongue
English
Weight
181 KB
Volume
68
Category
Article
ISSN
0006-3592

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โœฆ Synopsis

Displacement chromatography of proteins was successfully carried out in both hydrophobic interaction and reversed-phase chromatographic systems using low-molecular weight displacers. The displacers employed for hydrophobic displacement chromatography were water soluble, charged molecules containing several short alkyl and/or aryl groups. Spectroscopy was employed to verify the absence of structural changes to the proteins displaced on these hydrophobic supports. Displacement chromatography on a reversed-phase material was employed to purify a growth factor protein from its closely related variants, demonstrating the high resolutions that can be achieved by hydrophobic displacement chromatography. This process combines the high-resolution/high-throughput characteristics of displacement chromatography with the unique selectivity of these hydrophobic supports and offers the chromatographic engineer a powerful tool for the preparative purification of proteins.

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