Beta-galactosidase catalysed synthesis of branched oligosaccharide analogues
β Scribed by Stefan Menzler; Hasan Seker; Markus Gschrey; Manfred Wiessler
- Book ID
- 110369531
- Publisher
- Springer Netherlands
- Year
- 1997
- Tongue
- English
- Weight
- 175 KB
- Volume
- 19
- Category
- Article
- ISSN
- 0141-5492
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π SIMILAR VOLUMES
We describe a remote glycosidation that allows us to form a branched trisaccharide in intramolecular fashion. The molecular design of the spacer molecule for the remote glycosidation was examined based on molecular mechanics calculations.
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B-galactosidases of Thermus aquaticus YT-1, exhibiting a galactosyl transferase activity, were immobilized using different techniques. Entrapment in agarose or gellan gum beads was unsuitable for enzyme immobilization due to enzyme leakage. A technique that efficiently immobilized the enzymes was de