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Benzo(a)pyrene metabolism and DNA adduct formation in serially cultivated strains of human epidermal keratinocytes

✍ Scribed by E. Kenneth Parkinson; Robert F. Newbold

Publisher: John Wiley and Sons
Year: 1980
Tongue: French
Weight: 986 KB
Volume: 26
Category: Article
ISSN: 0020-7136
DOI: 10.1002/ijc.2910260307

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Six strains of human epidermal keratinocytes were shown to be capable of metabolising benzo(a)pyrene (BP)for at least 40 population doublings in culture. Metabolism was independent of human or mouse fibroblast products, and also the growth rate of the epidermal cultures, at least when cholera toxin and epidermal growth factor were included in the medium. Epidermal strains metabolized and bound to their DNA more BP than human fibroblasts, and themselves exhibited a 2‐to 3‐fold range of inter‐strain variability. Chromatog‐raphic analysis of epidermal DNA containing bound BP showed that the major metabolite which had reacted with the DNA was a 7,8 dihydrodiol ‐ 9,10 oxide of BP (the proposed ultimate carcinogenic metabolite of BP).

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