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Beneficial effects of embryo extract on cultured rabbit cartilage cells

✍ Scribed by Richard G. Ham; Louann W. Murray; Gerald L. Sattler

Publisher
John Wiley and Sons
Year
1970
Tongue
English
Weight
651 KB
Volume
75
Category
Article
ISSN
0021-9541

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✦ Synopsis

Abstract

Growth and the expression of cartilage‐like differentiation by adult rabbit chondrocytes is stimulated greatly by the Sephadex G‐25 macromolecular fraction of chicken embryo extract (CEEM). A small amount of fetal bovine serum or the macromolecular fraction of fetal bovine serum (FBSM) is also needed for growth. Medium F12 supplemented with CEEM plus FBSM supports moderate clonal growth of the rabbit chondrocytes with excellent expression of differentiated properties. The effects of CEEM on growth and the expression of differentiation have been quantitated by measurement of radioactive sulfate incorporation and total protein synthesis.

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