Behavior of Transforming Growth Factors in Serum-Free Media: An Improved Assay for Transforming Growth Factors
β Scribed by Angie Rizzino
- Book ID
- 125616894
- Publisher
- Society for In Vitro Biology
- Year
- 1984
- Tongue
- English
- Weight
- 776 KB
- Volume
- 20
- Category
- Article
- ISSN
- 1475-2689
- DOI
- 10.2307/4292886
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Mitogenic effects of fetal calf serum (FCS), plateletderived growth factor (PDGF), fibroblast growth factor (FGF), transforming growth factor-p (TGF-P), and forskolin to adult mouse Schwann cells were examined by bromodeoxyuridine (BrdU) incorporation and double immunofluorescence for SlOO and BrdU.
## Abstract Unlike untransformed 3T3 fibroblasts, Simian Virus 40βtransformed 3T3 cells (SV3T3) do not require the presence of exogenous polypeptide mitogens in order to become competent for the initiation of DNA synthesis. As an explanation for this state of perpetual competence, growth activity f