Biotinylated proteins as molecular weigh
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Dean Della-Penna; Rolf E. Christoffersen; Alan B. Bennett
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Article
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1986
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Elsevier Science
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English
โ 548 KB
Protein molecular weight standards were biotinylated by reaction with biotinyl-N-hydroxysuccinimide ester. The biotinylated proteins were resolved by sodium dodecyl sulfate-poiyacrylamide gel electrophoresis and electrophoretically transferred to nitrocellulose paper. The resolved protein bands were