Bayesian Analysis for the Meiosis I Non-disjunction Fraction in Numerical Chromosomal Anomalies

✍ Scribed by Rosangela H. Loschi; Glaura C. Franco

• Publisher: John Wiley and Sons
• Year: 2006
• Tongue: English
• Weight: 123 KB
• Volume: 48
• Category: Article
• ISSN: 0323-3847
• DOI: 10.1002/bimj.200510171

✦ Synopsis

Abstract

The main causes of numerical chromosomal anomalies, including trisomies, arise from an error in the chromosomal segregation during the meiotic process, named a non‐disjunction. One of the most used techniques to analyze chromosomal anomalies nowadays is the polymerase chain reaction (PCR), which counts the number of peaks or alleles in a polymorphic microsatellite locus. It was shown in previous works that the number of peaks has a multinomial distribution whose probabilities depend on the non‐disjunction fraction F . In this work, we propose a Bayesian approach for estimating the meiosis I non‐disjunction fraction F in the absence of the parental information. Since samples of trisomic patients are, in general, small, the Bayesian approach can be a good alternative for solving this problem. We consider the sampling/importance resampling technique and the Simpson rule to extract information from the posterior distribution of F . Bayes and maximum likelihood estimators are compared through a Monte Carlo simulation, focusing on the influence of different sample sizes and prior specifications in the estimates. We apply the proposed method to estimate F for patients with trisomy of chromosome 21 providing a sensitivity analysis for the method. The results obtained show that Bayes estimators are better in almost all situations. (© 2006 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)