The analysis of oligonucleotides using matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) has led to the investigation of the use of matrix additives (i.e., co-matrices) to help improve the poor spectral quality commonly observed during the analysis of this class of compounds.
Basic matrixes for the matrix-assisted laser desorption/ionization mass spectrometry of proteins and oligonucleotides
β Scribed by Fitzgerald, Michael C.; Parr, Gary R.; Smith, Lloyd M.
- Book ID
- 120447611
- Publisher
- American Chemical Society
- Year
- 1993
- Tongue
- English
- Weight
- 880 KB
- Volume
- 65
- Category
- Article
- ISSN
- 0003-2700
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Quantitative aspects of oligonucleotide analysis by matrix-assisted laser desorption/iotion (MALDI) mass spectrometry remain largely unexplored relative to the efforts that have been devoted to quantitative peptide and protein analysis. The successful quantitation of these other biopolymers coupled
Different buffers typically used in biological applications were evaluated for compatibility with matrix-assisted laser desorptiodionization m a s spectrometry (MALDI-MS) measurements. It was found that the buffers recommended by Good and co-workers, such as HEPES and MOPS in concentrations below 5