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Matrix Compatible Buffers for Analysis of Proteins with Matrix-assisted Laser Desorption/Ionization Mass Spectrometry

✍ Scribed by Ute Kallweit; K. Olaf Börnsen; Gerhard M. Kresbach; H. Michael Widmer

Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
506 KB
Volume
10
Category
Article
ISSN
0951-4198

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✦ Synopsis

Different buffers typically used in biological applications were evaluated for compatibility with matrix-assisted laser desorptiodionization m a s spectrometry (MALDI-MS) measurements. It was found that the buffers recommended by Good and co-workers, such as HEPES and MOPS in concentrations below 5 0 m ~ do not negatively affect the MALDI-MS measurements, a fact that is in contrast to phosphate buffers. Furthermore, increased peak intensities are observed in their presence as compared with pure water or other buffers. Additionally, buffer and salt concentrations higher than 1 0 0 m could be reduced by optimized purification methods, like drop dialysis or protein immobilization on transfer membranes.

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