Basic Laboratory Methods for Biotechnology: Textbook and Laboratory Reference

✍ Scribed by Lisa A. Seidman, Cynthia J. Moore, Jeanette Mowery

• Publisher: CRC Press
• Year: 2022
• Tongue: English
• Leaves: 1210
• Edition: 3
• Category: Library

✦ Synopsis

Basic Laboratory Methods for Biotechnology, Third Edition is a versatile textbook that provides students with a solid foundation to pursue employment in the biotech industry and can later serve as a practical reference to ensure success at each stage in their career. The authors focus on basic principles and methods while skillfully including recent innovations and industry trends throughout. Fundamental laboratory skills are emphasized, and boxed content provides step by step laboratory method instructions for ease of reference at any point in the students’ progress. Worked through examples and practice problems and solutions assist student comprehension. Coverage includes safety practices and instructions on using common laboratory instruments.

✦ Table of Contents

Contents
Preface
Ancillary Materials
Acknowledgements
Authors
UNIT I: Biotechnology Is the Transformation of Knowledge into Useful Products
1 Techniques to Manipulate DNA: The Root of the Biotechnology Industry
2 The Biotechnology Industry Branches Out
3 The Business of Biotechnology: The Transformation of Knowledge into Products
UNIT II: Introduction to Quality in Biotechnology Workplaces
4 An Overview of Quality Principles in Biotechnology
5 Quality in Research Laboratories
6 Documentation: The Foundation of Quality
UNIT III: Safety in the Laboratory
7 Introduction to a Safe Workplace
8 Working Safely in the Laboratory: General Considerations and Physical Hazards
9 Working Safely with Chemicals
10 Working Safely with Biological Materials
UNIT IV: Math in the Biotechnology Laboratory: An Overview
11 Basic Math Techniques
12 Proportional Relationships
13 Relationships and Graphing
14 Descriptions of Data: Descriptive Statistics
UNIT V: Obtaining Reproducible Laboratory Measurements
15 Introduction to Quality Laboratory Measurements
16 Introduction to Instrumental Methods and Electricity
17 The Measurement of Weight
18 The Measurement of Volume
19 The Measurement of Temperature
20 The Measurement of pH, Selected Ions, and Conductivity
21 Measurements Involving Light – Part A: Basic Principles and Instrumentation
UNIT VI: Laboratory Solutions
22 Preparation of Laboratory Solutions – Part A: Concentration Expressions and Calculations
23 Preparation of Laboratory Solutions – Part B: Basic Procedures and Buffers
24 Solutions: Associated Procedures and Information
25 Laboratory Solutions to Support the Activity of Biological Macromolecules
UNIT VII: Quality Assays and Tests
26 Introduction to Quality Laboratory Tests and Assays
27 Achieving Reproducible Results with Polymerase Chain Reaction Assays
28 Measurements Involving Light – Part B: Assays
29 Achieving Reproducible Immunoassay Results
UNIT VIII: Cell Culture and Reproducibility
30 Introduction to Quality Practices for Cell Culture
31 Culture Media for Intact Cells
UNIT IX: Basic Separation Methods
32 Introduction to Filtration
33 Introduction to Centrifugation
34 Introduction to Bioseparations
UNIT X: Biotechnology and Regulatory Affairs
35 Biotechnology and the Regulation of Medical and Food Products
36 The Lifecycles and Regulation of Pharmaceutical Products
37 The Lifecycles and Regulation of Biopharmaceutical and Regenerative Medicine Products
38 Quality Systems in a Regulated Production Facility
Appendix: Answers to Practice Problems
Acronyms
Glossary Terms
Index