Base-specific separation of oligodeoxynucleotides by capillary affinity gel electrophoresis
β Scribed by Professor Yoshinobu Baba; Tomohiro Sawa; Akio Kishida; Mitsuru Akashi
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 446 KB
- Volume
- 19
- Category
- Article
- ISSN
- 0173-0835
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We have developed an affinity capillary electrophoresis for gene mutation assay. We show a method for separating a mixture of oligodeoxynucleotides having single-base difference using oligodeoxynucleotide-polyacrylamide conjugate. Because the conjugate behaved similarly to nonionic polyacrylamide in
## Abstract Polyacrylamide gels are unstable in 50βΞΌm internal diameter capillaries at electric fields greater than about 500 V cm^β1^ due to the formation of bubbles, which destroy the separation. We describe the use of a modified polyacrylamide capillary gel that is stable at electric fields of a