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Barbiturate N-demethylase activity in isolated rat hepatocytes

✍ Scribed by Robert T. Abraham; Marion E. Sauers; Michael A. Zemaitis; John D. Alvin

Publisher
Elsevier Science
Year
1983
Tongue
English
Weight
817 KB
Volume
129
Category
Article
ISSN
0003-2697

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✦ Synopsis

The isolated hepatocyte preparation has become an increasingly popular system for studying xenobiotic metabolism, and there is a need for methods of determining metabolic activity. Described here is a simple and sensitive radiometric assay for barbiturate iV-demethylase activity in the isolated hepatocyte suspension. The demethylation of 1,3-dimethylphenobarbital is determined by measuring the formation of its only significant metabolite, N-methylphenobarbital. The reaction is a typical P-450-mediated dealkylation.

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