Flow cytometric analysis of xenobiotic metabolism activity in isolated rat hepatocytes
β Scribed by Kurt A. Black; Deborah L. Novicki; Jack L. Vincent; Gary J. Smith
- Publisher
- John Wiley and Sons
- Year
- 1993
- Tongue
- English
- Weight
- 506 KB
- Volume
- 14
- Category
- Article
- ISSN
- 0196-4763
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π SIMILAR VOLUMES
In the article by Kharbanda et al. (Flow Cytometric Analysis of Vesicular pH in Rat Hepatocytes After Ethanol Administration'' Vol. 26, No. 4,, the second line in the legend to Figure 4 reads ''25 Β΅mol/L ethanol.'' The correct unit of measure is ''25 mmole/L ethanol.''
We examined the effect of ethanol administration on intravesicular pH in intact hepatocytes by applying a flow cytometric technique to detect fluorescein-isothiocyanate-dextran (FITC-dextran) in acidic vesicles. Rats were pair-fed liquid diets containing either ethanol or isocaloric carbohydrate for
The development of hepatocyte polyploidy in rats aged up to 4 months was analyzed by flow cytometry using both scatter and fluorescent parameters to distinguish DNA diploid and DNA tetraploid populations and to discriminate between parenchymal and non-parenchymal compartments. The precise origin of