Bacteriophage Sigma Factor for RNA Polymerase
β Scribed by TRAVERS, ANDREW A.
- Book ID
- 109667965
- Publisher
- Nature Publishing Group
- Year
- 1969
- Tongue
- English
- Weight
- 447 KB
- Volume
- 223
- Category
- Article
- ISSN
- 0028-0836
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A structural gene for sigma factor (rpoD) of DNA-dependent RNA polymerase (RNA nucleotidyltransferase; nucleoside-triphosphate: RNA nucleotidyltransferase, E.C. 2.7.7.6) was mapped precisely by a set of F' factors including those already published (Proc. Natl. Acad. Sci. USA. 74, 1831-1835 (1977)).
Amber mutants of Escherichia coli K-12 affected in the structural gene (rpoD) for th a subunit ofRNA polymerase have been obtained from a strain harboring a temperature-sensitive amber suppressor (supF-Ts6) which is active only at low temperatures. These mutants grow normally at low temperature (30
Random mutagenesis of the gene for bacteriophage T7 RNA polymerase was used to identify functionally essential amino acid residues of the enzyme. A two-plasmid system was developed that permits the straightforward isolation of T7 RNA polymerase mutants that had lost almost all catalytic activity. It