Bacteriophage Mu-mediated gene transposition and in vitro cloning of the enterochelin gene cluster of Escherichia coli
โ Scribed by Laird, Alan J.; Ribbons, Douglas W.; Woodrow, Graeme C.; Young, Ian G.
- Book ID
- 123274516
- Publisher
- Elsevier Science
- Year
- 1980
- Tongue
- English
- Weight
- 775 KB
- Volume
- 11
- Category
- Article
- ISSN
- 0378-1119
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Phage Mu-1 mediated transposition has been used to map genes coding for ribosomal proteins and elongation factor G inside transcriptional units. The data indicate that 1) the str A and fusA genes belong to the same operon, 2) the spcA and strA genes are expressed independently, 3) the spcA gene is l
An Escherichia coli mutant has been isolated (Hek) in which the kil gene of bacteriophage Mu is not functionally expressed. The hek locus has been mapped between rpoD (66.2 min) and argR (69.5 min) on the E. coli chromosome. No influence of the hek mutation on phage or E. coli development could be d