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Bacteriophage degradation of the capsular polysaccharide from Klebsiella K69: Location of the O-acetyl groups

✍ Scribed by P.Linton Hackland; Haralambos Parolis; Anne Dell; Philip R. Tiller

Publisher: Elsevier Science
Year: 1988
Tongue: English
Weight: 568 KB
Volume: 181
Category: Article
ISSN: 0008-6215
DOI: 10.1016/0008-6215(88)84031-x

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✦ Synopsis

The 0-acetyl group in the repeating unit of the capsular polysaccharide of Klebsiella K69 has been located on O-6 of the 4-linked j?-mannosyl residue by a combination of *H-and 13C-n.m.r. spectroscopy and f.a.b.-m.s. of the pentasaccharide obtained by degradation of the polysaccharide with a bacteriophage. Only every third repeating-unit in the polysaccharide chain is 0-acetylated. Phage @69 was shown to be a p-D-mannosidase and cleaved the P-D-Manp-(l-+4)-B-D-Glcp linkages in the polysaccharide.

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