added to the dsDNA before hybridization. The color of the solution in test reaction was compared with the set of standards and the presence of dsDNA was determined. The setup is able to detect picogram level of dsDNA and colorimetric method is found tenfold more sensitive then visualized under UV tr
✦ LIBER ✦
Bacterial type IV secretion systems: a tool for DNA transfer into mammalian cells
✍ Scribed by E. Fernández-González; H.D. de Paz; C. Dehio; F.J. Sangari; M. Llosa
- Publisher
- Elsevier
- Year
- 2009
- Tongue
- English
- Weight
- 69 KB
- Volume
- 25
- Category
- Article
- ISSN
- 1871-6784
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Background Tight control of gene activity has been achieved in cells and transgenic organisms using the Tet regulatory systems. Unregulated basal transcription can, however, be observed whenever integration of target genes driven by promoters responsive to tetracycline controlled transcriptional act