Bacterial Spore Detection and Determination by Use of Terbium Dipicolinate Photoluminescence
โ Scribed by Rosen, David L.; Sharpless, Charles; McGown, Linda B.
- Book ID
- 120369256
- Publisher
- American Chemical Society
- Year
- 1997
- Tongue
- English
- Weight
- 177 KB
- Volume
- 69
- Category
- Article
- ISSN
- 0003-2700
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
Dipicolinic acid was extracted from approximately 0.1 mg spores or 0.5 ml of sporulating culture with 20 mM HCl for 10 min at 100 degrees C. The suspension was diluted with 5 mM Ca2+, 100 mM Tris, pH 7.6, centrifuged, and the first derivative of the uv absorbance spectrum recorded from 275 nm to 285
The occurrence of substantial amounts of dipicolinic acid (DPA) in bacterial spores, the idea that DPA is related in some yet-to-be-explained way to the extraordinary heat stability of these cells, and the lack of knowledge of any function for this biologically unusual compound make a rapid accurate