Bacterial Pathogenesis

✍ Scribed by Peter Williams, Julian Ketley and George Salmond (Eds.)

Publisher: Academic Press
Year: 1998
Tongue: English
Leaves: 643
Series: Methods in Microbiology 27
Edition: 1st
Category: Library

No coin nor oath required. For personal study only.

✦ Synopsis

Established almost 30 years ago, Methods in Microbiology is the most prestigious series devoted to techniques and methodology in the field. Now totally revamped, revitalized, with a new format and expanded scope, Methods in Microbiology will continue to provide you with tried and tested, cutting-edge protocols to directly benefit your research. Key Features * Focuses on the methods most useful for the microbiologist interested in the way in which bacteria cause disease * Includes section devoted to 'Approaches to characterising pathogenic mechanisms' by Stanley Falkow * Covers safety aspects, detection, identification and speciation * Includes techniques for the study of host interactions and reactions in animals and plants * Describes biochemical and molecular genetic approaches * Essential methods for gene expression and analysis * Covers strategies and problems for disease control

✦ Table of Contents

Content:
Series Advisors
Page ii

Edited by
Page iii

Copyright page
Page iv

Contributors
Pages ix-xiv

Preface
Pages xv-xix
Peter Williams, Julian Ketley, George Salmond

1.1 Detection of Virulence Genes Expressed within Infected Cells Original Research Article
Pages 3-12
Raphael H. Valdivia, Stanley Falkow

2.1 Introduction Original Research Article
Pages 15-17
Paul V. Tearle

2.2 Safe Working Practices in the Laboratory - Safe Person Strategy Original Research Article
Pages 19-28
Paul V. Tearle

2.3 Safe Working Practices in the Laboratory - Safe Place Strategy Original Research Article
Pages 29-35
Paul V. Tearle

3.1 Introduction Original Research Article
Page 39
Peter Vandamme

3.2 Detection Original Research Article
Pages 41-50
Margareta Ieven

3.3 Speciation Original Research Article
Pages 51-56
Peter Vandamme

3.4 Identification Original Research Article
Pages 57-65
Peter Vandamme

4.1 Introduction Original Research Article
Pages 69-71
T.J. Mitchell

4.2 Interaction of Bacteria and their Products with Tissues in Organ Culture Original Research Article
Pages 73-81
Alan D. Jackson, Ruth B. Dowling, Robert Wilson

4.3 Transgenic and Knockout Animals in the Study of Bacterial Pathogenesis Original Research Article
Pages 83-102
Bernard Burke

4.4 Interactions of Bacteria and their Products in Whole Animal Systems Original Research Article
Pages 103-112
Timothy S. Wallis

4.5 Interaction of Bacteria and their Products with Tissue Culture Cells Original Research Article
Pages 113-122
Darlene Charboneau, Jeffrey B. Rubins

5.1 Introduction Original Research Article
Pages 125-128
Michael J. Daniels

5.2 Testing Pathogenicity Original Research Article
Pages 129-137
Michael J. Daniels

5.3 hrp Genes and Their Function Original Research Article
Pages 139-148
Alan Collmer, Steven V. Beer

5.4 Avirulence Genes Original Research Article
Pages 149-155
Ulla Bonas

5.5 Extracellular Enzymes and Their Role in Erwinia Virulence Original Research Article
Pages 157-168
B. Py, F. Barras, S. Harris, N. Robson, G.P.C. Salmond

5.6 Bacterial Phytotoxins Original Research Article
Pages 169-175
Carol L. Bender

5.7 Epiphytic Growth and Survival Original Research Article
Pages 177-182
Steven E. Lindow

6.1 Introduction: Fractionation of Bacterial Cell Envelopes Original Research Article
Pages 185-190
Kim Hardie, Paul Williams

6.2 The Proteome Approach Original Research Article
Pages 191-204
C. David O'Connor, Michele Farris, Lawrence G. Hunt, J. Neville Wright

6.3 Microscopic Approaches to the Study of Bacterial Cell-Associated Virulence Determinants Original Research Article
Pages 205-214
Nicholas B.L. Powell

6.4 Characterization of Bacterial Surface Receptor-Ligand Interactions Original Research Article
Pages 215-225
Julie Holland, Andrew Gorringe

6.5 Characterizing Flagella and Motile Behavior Original Research Article
Pages 227-238
R. Elizabeth Sockett

6.6 Fimbriae: Detection, Purification, and Characterization Original Research Article
Pages 239-248
Per Klemm, Mark Schembri, Bodil Stentebjerg-Olesen, Henrik Hasman, David L. Hasty

6.7 Isolation and Purification of Cell Surface Polysaccharides from Gram-Negative Bacteria Original Research Article
Pages 249-258
Chris Whitfield, Malcolm B. Perry

6.8 Structural Analysis of Cell Surface Polysaccharides from Gram-Negative Bacteria Original Research Article
Pages 259-275
Malcolm B. Perry, Chris Whitfield

6.9 Techniques for Analysis of Peptidoglycans Original Research Article
Pages 277-286
Kevin D. Young

6.10 Bacterial Exotoxins Original Research Article
Pages 287-300
Mahtab Moayeri, Rodney A. Welch

6.11 A systematic approach to the study of protein secretion in gram-negative bacteria Original Research Article
Pages 301-318
Alain Filloux, Kim R. Hardie

6.12 Detection, Purification, and Synthesis of n-acylhomoserine Lactone Quorum Sensing Signal Molecules Original Research Article
Pages 319-330
Miguel C`mara, Mavis Daykin, Siri Ram Chhabra

6.13 Iron Starvation and Siderophore-Mediated iron Transport Original Research Article
Pages 331-342
Anthony W. Smith

7.1 Introduction Original Research Article
Pages 345-347
Charles J. Dorman

7.2 Genetic Approaches to the Study of Pathogenic Salmonellae Original Research Article
Pages 349-357
Jay C.D. Hinton

7.3 Signature Tagged Mutagenesis Original Research Article
Pages 359-370
David W. Holden, Michael Hensel

7.4 Physical Analysis of the Salmonella Typhimurium Genome Original Research Article
Pages 371-381
Shu-Lin Liu, Kenneth E. Sanderson

7.5 Molecular Analysis of Pseudomonas Aeruginosa Virulence Original Research Article
Pages 383-393
H. Yu, J.C. Boucher, V. Deretic

7.6 Molecular Genetics of Bordetella Pertussis Virulence Original Research Article
Pages 395-406
Vincenzo Scarlato, Dagmar Beier, Rino Rappuoli

7.7 Genetic Manipulation of Enteric Campylobacter Species Original Research Article
Pages 407-419
Arnoud H.M. Van Vliet, Anne C. Wood, Karl Wooldridge, Julian M. Ketley, John Henderson

7.8 Molecular Approaches for the Study of Listeria Original Research Article
Pages 421-431
Silke Schäferkordt, Eugen Domann, Trinad Chakraborty

7.9 Molecular Genetic Analysis of Staphylococcal Virulence Original Research Article
Pages 433-454
T.J. Foster

7.10 Molecular Approaches to Studying Chlamydia Original Research Article
Pages 455-464
Marci A. Scidmore, John Bannantine, Ted Hackstadt

8.1 Gene Expression and Analysis Original Research Article
Pages 467-483
Ian Roberts

9.1 Introduction Original Research Article
Pages 487-489
Mark Roberts

9.2 Strategies for the Study of Interactions Between Bacterial Adhesins and Glycosaminoglycans Original Research Article
Pages 491-498
Franco D. Menozzi, Camille Locht

9.3 Cytoskeletal Rearrangements Induced by Bacterial Pathogens Original Research Article
Pages 499-507
Ilan Rosenshine

9.4 Activation and Inactivation of Ras-Like Gtpases by Bacterial Cytotoxins Original Research Article
Pages 509-525
Christoph von Eichel-Streiber, Manfred Weidmann, Murielle Giry, Michael Moos

9.5 Strategies for the use of Gene Knockout Mice in the Investigation of Bacterial Pathogenesis and Immunology Original Research Article
Pages 527-535
Paul Everest, Gordon Dougan, Mark Roberts

10.1 Host Reactions - Plants Original Research Article
Pages 539-566,566a,567-572
Charles S. Bestwick, Ian R. Brown, John W. Mansfield, Bernard Boher, Michel Nicole, Margaret Essenberg

11.1 Introduction Original Research Article
Pages 575-576
Alejandro Cravioto

11.2 Strategies for Control of Common Infectious Diseases Prevalent in Developing Countries Original Research Article
Pages 577-586
Alejandro Cravioto, Carlos Eslava, Yolanda Lopez-Vidal, Roberto Cabrera

11.3 Antibiotic Resistance and Prescription Practices in Developing Countries Original Research Article
Pages 587-594
Carlos F. Amábile-Cuevas, Roberto Cabrera, Fermín Valenzuela, L. Yolanda Fuchs

12.1 Internet Resources for Research on Bacterial Pathogenesis Original Research Article
Pages 597-606
Mark Pallen

Index
Pages 607-620

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