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Bacterial lipases: an overview of production, purification and biochemical properties

✍ Scribed by R. Gupta; N. Gupta; P. Rathi


Book ID
105949668
Publisher
Springer
Year
2004
Tongue
English
Weight
229 KB
Volume
64
Category
Article
ISSN
1432-0614

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A white-rot fungus Dichomitus squalens, when grown on 1% wheat-straw glucuronoarabinoxylan under aerated submerged conditions, secreted an a-~arabinofuranosidase (4.3 nkat/mL). The enzyme was purified 70-fold by ammonium sulfate precipitation, chromatofocusing on PBE 94, gel filtration on Ultrogel A

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## Abstract Bacterial acyltransferases are a new class of enzymes, and the first member was identified as WS/DGAT in __Acinetobacter baylyi__ ADP1. Their unspecificity have been used in several biotechnological applications for lipid modification, a field that has been dominated by the use of lipas