## Abstract A strain of __Pseudomonas mendocina__ producing extracellular lipase was isolated from soil. The bacterium accumulates lipase in culture fluid when grown aerobically at 30 Β°C for 24 h in a medium composed of olive oil (1%) as substrate. __Pseudomonas mendocina__ lipase was optimally act
Production and properties of an alkaline, thermophilic lipase from Pseudomonas fluorescens NS2W
β Scribed by N Kulkarni; R V Gadre
- Book ID
- 110050950
- Publisher
- Springer-Verlag
- Year
- 2002
- Tongue
- English
- Weight
- 104 KB
- Volume
- 28
- Category
- Article
- ISSN
- 1476-5535
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## Abstract The extracellular endopeptidase synthesized by soil bacterium __Pseudomonas fluorescens__ was purified to homogeneity in a fourβstep procedure. The enzyme was purified 45βfold, with a 20% recovery. The endopeptidase appeared to be a monomer with a molecular mass of approx. 50 kDa. The e
## Abstract **BACKGROUND:** The alkaline lipase production on vegetable oils as sole carbon source, its characterization and effect of different commercial detergents and surfactants on enzymatic activity from thermophilic __Bacillus__ sp. DH4 was investigated. **RESULTS:** The organism grew on ma