Bacterial degradation of p-methoxybenzoic acid

✍ Scribed by Buswell, J. A. ;Mahmood, A.

• Publisher: Springer-Verlag
• Year: 1972
• Weight: 732 KB
• Volume: 84
• Category: Article
• ISSN: 0003-9276
• DOI: 10.1007/bf00409077

✦ Synopsis

A ceIl-fi'ee system from a Pseudomonas sp., strain P~3, catalysed the oxidative demethylation, hydroxylation and subsequent ring cleavage of p-methoxybenzoate. Demethylation, to yield p-hydroxybenzoate, involved absorption of 1.0 mole of oxygen/mole of p-methoxybenzoate, and required reduced pyridine nucleotide (either NADH or NADPH) as cofacter, p-Hydroxybenzoate was hydroxylated to yield protocatechuate with the absorption of 1 mole of oxygen/mole of substrate, and required NADPH as cofacter. Protocafechuate was oxidized, with absorption of 1 mole of oxygen/mole of substrate, to 3-oxoadipate. The methyl group of p-methoxybenzoate was removed as formaldehyde, and oxidized to formate and carbon dioxide by formaldehyde dchydrogenase, which required GSH and NAD +, and formate dehydrogenase, which required NAD+.