Bacterial 2,3-butanediol dehydrogenases

✍ Scribed by Hanni Höhn-Bentz; F. Radler

• Book ID: 104760449
• Publisher: Springer
• Year: 1978
• Tongue: English
• Weight: 613 KB
• Volume: 116
• Category: Article
• ISSN: 0302-8933
• DOI: 10.1007/bf00406037

✦ Synopsis

Enterobacter aerogenes, Aeromonas hydrophilao Serratia marcescens and Staphylococcus aureus possessing L(+)-butanediol dehydrogenase produced mainly meso-butanediol and small amounts of optically active butanediol; Acetobacter suboxydans, Ba- cillus polymyxa and Erwinia carotovora containing D(-)butanediol dehydrogenase produced more optically active butanediol than meso-butanediol. Resting and growing cells of these organisms oxidized only one enantiomer of racemic butanediol. The D(-)-butanediol dehydrogenase from Bacillus poIymyxa was partially purified (30-fold) with a specific activity of 24.5. Except NAD and N A D H no other cofactors were required. Optimum pH-values for oxidation and reduction were pH 9 and pH 7, respectively. The optimum temperature was about 60 ~ C.