𝔖 Bobbio Scriptorium
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Bacteria-Based Biosensor for Determination of Hydrogen Peroxide

✍ Scribed by G.Y. Tai; M.L. Wen; C.Y. Wang

Publisher
Elsevier Science
Year
1996
Tongue
English
Weight
73 KB
Volume
53
Category
Article
ISSN
0026-265X

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✦ Synopsis

A bacterial biosensor that immobilizes living bacteria of strain Bacillus subtilis AS 1.398 are coupled to a Clark-type oxygen electrode to determine hydrogen peroxide. The H 2 O 2 is catalyzed by catalase from the bacteria to produce H 2 O and O 2 . This sensor is advantageous in terms of linearity (10 to 2000 M H 2 O 2 ), lifetime (65 days), and selectivity (no significant interference from some amino acids, metal ions, polyphenols, glucose, and ascorbic acid), especially because bacteria can be used as 2 catalase enzyme membrane that can reproduce itself. The correlation coefficient is 0.999.

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