Properties of an inversion and a deletion mutant of B. subtilis phage SPP1 which arose during cloning are described. The results are related to the biology of this bacteriophage. In preceding communications from our laboratories (Heilmann and Reeve 1982, Behrens et al. 1983) we reported the properti
Bacillus subtilis bacteriophage SPβc1 is a deletion mutant of SPβ
✍ Scribed by Fink, P. S. ;Korman, R. Z. ;Odebralski, J. M. ;Zahler, S. A.
- Publisher
- Springer
- Year
- 1981
- Tongue
- English
- Weight
- 214 KB
- Volume
- 182
- Category
- Article
- ISSN
- 0026-8925
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✦ Synopsis
The restriction fragment patterns of two mutants forms of the temperate Bacillus subtilis bacteriophage SP beta have been examined. The DNA of a heat-inducible mutant, SP beta c2, which has a molecular size of 128 kilobases (kb), yields the same restriction pattern as the wild type SP beta c+ DNA. The DNA of a clear-plaque mutant, SP beta c1, has a molecular size of 117 kb, and is deleted for an 11 kb region of phage DNA. Neither SP beta c1 nor SP beta c2 DNA is cleaved by the endonuclease HaeIII.
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