B16 melanoma cell spreading on activated endothelial cells
β Scribed by Yoshito Sadahira; Tadashi Yoshino; Naoya Kojima
- Book ID
- 118292949
- Publisher
- Society for In Vitro Biology
- Year
- 1994
- Tongue
- English
- Weight
- 212 KB
- Volume
- 30
- Category
- Article
- ISSN
- 1475-2689
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The role of mannose receptors from hepatic sinusoidal endothelium (HSE) in liver colonization by B16 melanoma (B16M) cells was studied. The expression of high mannose-type oligosaccharides on the surface of B16M cells was enhanced by in vitro treatment with 1-deoximannojirimycin (1-DMM). There was a
## Abstract Liverβcolonizing tumor cells were selected from Bβ16 melanoma by a stepwise procedure: (1) injection of the original cells through the portal circulation; (2) removal of tumor cells from the hepatic tumor nodules for culture __in vitro__; (3) reinjection of such cells through the portal