Hybridomas were derived from lipopolysaccharide-reactive splenic B cells of adult germ-free BALB/c mice fed a chemically defined ultrafiltered "antigen-free'' diet (GF-CD) and from splenic B cells of 5-day-old conventional (CV-NEO) BALBic mice. The monoclonal antibodies (mAb) from both collections o
B cell repertoire in adult antigen-free and conventional neonatal balb/c mice
โ Scribed by Nicolaas A. Bos; Cornelia G. Meeuwsen
- Publisher
- John Wiley and Sons
- Year
- 1989
- Tongue
- English
- Weight
- 730 KB
- Volume
- 19
- Category
- Article
- ISSN
- 0014-2980
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A large number of hybridomas were constructed by fusion of B cells from perinatal liver and spleen. Many of these showed multispecificity, high interconnectivity and anti-idiotype (Id) activity. Several of these were subjected to a detailed analysis to evaluate their influence on the developing immu