Axonal transport of synaptic vesicle proteins in the rat optic nerve

✍ Scribed by Li, Jia-Yi ;Dahlstr�m, Annica

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 642 KB
• Volume: 32
• Category: Article
• ISSN: 0022-3034
• DOI: 10.1002/(sici)1097-4695(199702)32:2<237::aid-neu8>3.0.co;2-9

✦ Synopsis

the sciatic nerve. Most synaptic vesicle proteins were colo-ABSTRACT: The optic nerve, as a part of the cencalized in the axons proximal to the crush. A differential tral nervous system (CNS), has been used to study axonal distribution of synaptobrevin I and II, however, was obtransport for decades. The present study has concentrated on the axonal transport of synaptic vesicle proteins served in the optic nerve axons; synaptobrevin I was in the optic nerve, using the ''stop-flow/nerve crush'' present in large-sized axons, while synaptobrevin II immethod. After blocking fast axonal transport, distinct acmunoreactivity was present in most axons, including the cumulations of synaptic vesicle proteins developed during large ones. The two isoforms were, thus, partially colocalthe first hour after crush-operation and marked increases ized. The results demonstrate that (1) cytofluorimetric were observed up to 8 h postoperative. Semiquantitative scanning techniques could be successfully used to study analysis, using cytofluorimetric scanning (CFS) of immuaxonal transport not only in peripheral nerves, but also noincubated sections, revealed that the ratio between disin the CNS; (2) synaptic vesicles are transported with tal accumulations (organelles in retrograde transport) fast axonal transport in this nerve; and (3) some differand proximal accumulations (organelles in anterograde ences were noted compared with the sciatic nerve, espetransport) was much higher (up to 80-90%) for the cially for Rab3a and clathrin. ᭧ 1997 John Wiley & Sons, Inc. transmembrane proteins than that for surface adsorbed J Neurobiol 32: 237-250, 1997

proteins (only 10-20%). The pattern of axonal transport

Keywords: synaptic vesicle proteins; axonal transport; in the optic nerve was comparable to that in the sciatic cytofluorimetric scanning; immunofluorescence; optic nerve. However, clathrin and Rab3a immunoreactivities nerve; rat were accumulated in much lower amounts than that in