✦ LIBER ✦

Avidin. II. Composition and mode of action of avidin A

✍ Scribed by H. Fraenkel-Conrat; N.S. Snell; E.D. Ducay

Book ID: 118839509
Publisher: Elsevier Science
Year: 1952
Tongue: English
Weight: 618 KB
Volume: 39
Category: Article
ISSN: 0003-9861
DOI: 10.1016/0003-9861(52)90264-6

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Underestimation of avidin and streptavidin by the Bradford method can be alleviated by carrying out the reaction at 100 degrees C for 10-15 min, and the protein values thus obtained match very well with those of biotin-binding assays. "Thermal modification" is applicable to "standard" and "micro" ve