𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Auxotrophic mutants of the yeast Trichosporon adeninovorans

✍ Scribed by Ida A. Samsonova; Prof. Dr. Fritz Böttcher; Carsta Werner; Rüdiger Bode

Publisher
John Wiley and Sons
Year
1989
Tongue
English
Weight
527 KB
Volume
29
Category
Article
ISSN
0233-111X

No coin nor oath required. For personal study only.

✦ Synopsis

We have isolated and characterized auxotrophic mutants of Trichosporon adeninovorans, strain PAR-4 to get genetic markers that cover the entire nuclear genome of this thermotolerant yeast of technological interest. The nitrosoguanidine mutagenesis yielded mutants at a high frequency. We detected a broad spectrum of auxotrophic phenotypes in the random mutant samples. Obviously, strain PAR-4 is a haploid-or hyperhaploid yeast. In correspondence we determined a low DNA content per cell. In contrast to NG'), UV light was an inefficient mutagen. UV survival curves were without the typical shoulder indicating suppression of repair of UV-induced lethal lesions. Thus, the response of PAR-4 to UV was different from those of Saccharomyces cerevisiae and other yeasts.

')

📜 SIMILAR VOLUMES

Purification and characterization of the
Purification and characterization of the extracellular glucoamylase from the yeast Trichosporon adeninovorans
✍ R. Büttner; Dr. R. Bode; D. Birnbaum 📂 Article 📅 1987 🏛 John Wiley and Sons 🌐 English ⚖ 581 KB

The yeast Trichosporon adeninovorans secretes an amylase a t a high rate if grown in a medium containing starch or maltose. The enzyme was purified 29-fold by hydroxylapatite chromatography and gel filtration and characterized as a glucoamylase. The enzyme seems to be a glycoprotein with a molecular

Isolation and characterization of mutant
Isolation and characterization of mutants of Trichosporon adeninovorans resistant to 2-deoxy-D-glucose
✍ Dr. R. Büttner; A. Scheit; R. Bode; D. Birnbaum 📂 Article 📅 1989 🏛 John Wiley and Sons 🌐 English ⚖ 346 KB

We isolated stable mutants from Trichosporon adeninovorans resistant to 2-deoxy-~-glucose. All mutants were altered in the C-catabolite repression and produced different amounts of glucoamylase and 8-glucosidase when grown on several carbon sources. In addition to the C-catabolite derepression of th

Mapping of the glucoamylase gene of Tric
Mapping of the glucoamylase gene of Trichosporon adeninovorans by mitotic haploidization using hybrids from protoplast fusions
✍ Dr. R. Büttner; R. Bode; I. A. Samsonova; D. Birnbaum 📂 Article 📅 1990 🏛 John Wiley and Sons 🌐 English ⚖ 280 KB

In two strains of Trichosporon adeninovorans genes of unknown location were assigned to linkage groups. Diploid strains were produced by protoplast fusion and partially haploidized by growth on benomyl causing mitotic haploidization. We show that both strains synthesize an extracellular glucoamylase

Differential fates of invertase mutants
Differential fates of invertase mutants in the yeast endoplasmic reticulum
✍ McCracken, Ardythe A.; Werner, Eric D.; Powell, Marguerite J.; Kruse, Kristina B 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 111 KB

A number of proteins have been identi®ed as substrates for endoplasmic reticulum (ER)-associated protein degradation (ERAD) and we describe here a new model substrate with which to study this process. Two secretiondefective forms of yeast invertase that accumulated in the ER to greatly different lev

Auxotrophic mutant of the cyanobacterium
Auxotrophic mutant of the cyanobacterium Nostoc muscorum showing absolute requirement of Cs+ or Rb+ for diazotrophy and autotrophy
✍ Santosh Bhargava; Rajendra Chauhan; Pramod K. Patil; Sarva Daman Singh 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 80 KB

## Abstract Caesium‐resistant (Cs^+^‐R) mutant clones of the cyanobacterium __Nostoc muscorum__ were characterized for diazotrophic growth in a medium devoid of Cs^+^ or Rb^+^ or both. Cs^+^‐R phenotype suffered severe genetic damage of a pleiotropic nature affecting diazotrophic growth, chlorophyl

Action of FdUrd and dCyd on the incorpor
Action of FdUrd and dCyd on the incorporation of brdurd in Chinese hamster somatic cell DNA and the isolation of auxotrophic mutants
✍ Romain Labrecque; Jean-Paul Thirion 📂 Article 📅 1977 🏛 John Wiley and Sons 🌐 English ⚖ 435 KB

## Abstract Chinese hamster somatic cells grown in the presence of bromodeoxyuridine, deoxycytidine and fluorodeoxyuridine incorporate more bromodeoxyuridine in their DNA than cells grown in the presence of bromodeoxyuridine alone. Thus they become more sensitive to light irradiation. Our data sugg