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Autoradiography using storage phosphor technology

✍ Scribed by Richard F. Johnston; Siobhan C. Pickett; Dr. David L. Barker

Book ID: 102833756
Publisher: John Wiley and Sons
Year: 1990
Tongue: English
Weight: 652 KB
Volume: 11
Category: Article
ISSN: 0173-0835
DOI: 10.1002/elps.1150110503

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✦ Synopsis

We describe the application of photostimulable storage phosphor imaging plates to autoradiography of sampleslabeled with 32P, I4C and 35S. Imaging plates can detect a 32P-labeled sample at an exposurelevel of 1 disintegration/mm2, and I4C and 35S at 25 disintegrations/mm*. Compared to X-ray film, imaging plates offer increased sensitivity of 15-to 250-fold for 32P and 20-to 100-fold for 14C and 35S. Resolution is in the 0.3 mm range and is therefore suitable for any gel or blot application.

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