Autoradiographic visualization of beta adrenergic receptors in fish melanophores

✍ Scribed by Komatsu, Koichiro ;Yamada, Koji

• Publisher: John Wiley and Sons
• Year: 1982
• Tongue: English
• Weight: 406 KB
• Volume: 223
• Category: Article
• ISSN: 0022-104X
• DOI: 10.1002/jez.1402230211

✦ Synopsis

Abstract

Autoradiographic demonstration of beta adrenergic receptors was achieved light microscopically in melanophores of isolated scales of a teleost fish, Oryzias latipes. [^3^H]hydroxybenzylisoproterenol, a beta adrenergic agonist, and [^3^H]propranolol, a beta adrenergic antagonist, were used as radioligands. In the autoradiograms, photographic grains of considerably high density were found to be distributed evenly over all parts of the surface of melanophores. Since the density of the grains was observed to decrease significantly in the presence of excess amounts of nonlabeled ligands, the results indicate the actual existence of beta adrenergic receptors in the present melanophores. This substantiates the findings obtained by other physiological and pharmacological methods that beta‐adrenergic receptors may exist and be involved in melanosome‐dispersion response of fish melanophores.